Hotspot

rps16-trnQ_UUG

LSC · 5573-7026 · 1454 bp

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Pi 0.0429
MarkerSeek score 74.40
Primer available yes
Label rps16-trnQ_UUG

Feature Metadata

label_name
rps16-trnQ_UUG
feature_type
igs
region
LSC
length_bp
1454
variable_sites
710
pi
0.0429
unique_haplotype_count
189
primer_available
yes
alignment_reliability
0.79
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_UUG_p1 TATGTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 351-1600 bp 0.920705 88.1
rps16-trnQ_UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 341-1590 bp 0.942731 88.0
rps16-trnQ_UUG_p3 TGGCGGATGTAAGAATCCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 577-1627 bp 0.894273 84.6
rps16-trnQ_UUG_p4 GGCGGATGTAAGAATCCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 576-1626 bp 0.889868 84.4
rps16-trnQ_UUG_p5 TATGTCCTTCAAGTCGCACG TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.0 57.3 355-1604 bp 0.920705 83.4