Hotspot

ycf1

SSC · 129353-134659 · 5307 bp

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Pi 0.00369
MarkerSeek score 36.40
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5307
variable_sites
48
pi
0.00369
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACAAGAGGTCTTTCAAACCAGA TGAGCGAAACCTCAATAGAA 40.9 40.0 57.5 54.4 211 bp 1.0 42.7
ycf1_p2 ACAAGAGGTCTTTCAAACCAGA TGAGCGAAACCTCAATAGAAA 40.9 38.1 57.5 55.3 211 bp 1.0 42.7
ycf1_p3 ACAAGAGGTCTTTCAAACCAGA ATGAGCGAAACCTCAATAGA 40.9 40.0 57.5 54.2 212 bp 1.0 42.6
ycf1_p4 GGTTCCACCGTTGATAATCA TGAGCGAAACCTCAATAGAA 45.0 40.0 55.4 54.4 243 bp 1.0 42.3
ycf1_p5 GGTTCCACCGTTGATAATCA TGAGCGAAACCTCAATAGAAA 45.0 38.1 55.4 55.3 243 bp 1.0 42.3