Pi
0.00133
MarkerSeek score
51.10
Primer available
yes
Label
rpl16
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpl16_p1 | TCAATGCAAAGAGTGAAGGA | TCGATTGACCATTTCATTTGG | 40.0 | 38.1 | 54.9 | 54.8 | 1556-1571 bp | 1.0 | 40.9 |
| rpl16_p2 | TCAATGCAAAGAGTGAAGGAA | TCGATTGACCATTTCATTTGG | 38.1 | 38.1 | 55.7 | 54.8 | 1556-1571 bp | 1.0 | 40.9 |
| rpl16_p3 | TTCAATGCAAAGAGTGAAGGA | TCGATTGACCATTTCATTTGG | 38.1 | 38.1 | 55.7 | 54.8 | 1557-1572 bp | 1.0 | 40.9 |
| rpl16_p4 | TCAATGCAAAGAGTGAAGGA | TTCGATTGACCATTTCATTTGG | 40.0 | 36.4 | 54.9 | 55.6 | 1557-1572 bp | 1.0 | 40.9 |
| rpl16_p5 | CTTTCAATGCAAAGAGTGAAGGA | TCGATTGACCATTTCATTTGG | 39.1 | 38.1 | 57.6 | 54.8 | 1559-1574 bp | 1.0 | 40.9 |