Hotspot

petD

LSC · 81112-82385 · 1274 bp

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Pi 0.00381
MarkerSeek score 51.30
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1274
variable_sites
16
pi
0.00381
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 TTTCTCTTCAACCCCGAAGT TACCCATTATGCCGCGTTAC 45.0 50.0 56.7 58.1 1393-1401 bp 1.0 69.3
petD_p2 TTTCTCTTCAACCCCGAAGT GATACCCATTATGCCGCGTT 45.0 50.0 56.7 58.5 1395-1403 bp 1.0 66.5
petD_p3 ATTTCTCTTCAACCCCGAAGT TACCCATTATGCCGCGTTAC 42.9 50.0 57.2 58.1 1394-1402 bp 1.0 65.5
petD_p4 TTTCTCTTCAACCCCGAAGT CATTATGCCGCGTTACAGTC 45.0 50.0 56.7 57.4 1389-1397 bp 1.0 65.2
petD_p5 TTTCTCTTCAACCCCGAAGT CCGCGTTACAGTCGAATCAA 45.0 50.0 56.7 58.7 1382-1390 bp 1.0 65.1