Hotspot

ndhC

LSC · 54554-54916 · 363 bp

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Pi 0
MarkerSeek score 26.80
Primer available yes
Label ndhC

Feature Metadata

label_name
ndhC
feature_type
gene
region
LSC
length_bp
363
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC_p1 TGGAGATTGTTTGGTCAACT TCGAATCGACAATTTCAATTTGGA 40.0 33.3 54.8 57.8 520 bp 1.0 40.0
ndhC_p2 TTGGAGATTGTTTGGTCAACT TCGAATCGACAATTTCAATTTGGA 38.1 33.3 55.6 57.8 521 bp 1.0 40.0
ndhC_p3 TGGAGATTGTTTGGTCAACT CGAATCGACAATTTCAATTTGGA 40.0 34.8 54.8 56.4 519 bp 1.0 40.0
ndhC_p4 TGGAGATTGTTTGGTCAACT TCGAATCGACAATTTCAATTTGG 40.0 34.8 54.8 56.4 520 bp 1.0 40.0
ndhC_p5 CTGAATTGGAGATTGTTTGGTCA TCGAATCGACAATTTCAATTTGGA 39.1 33.3 57.3 57.8 526 bp 1.0 40.0