Hotspot

clpP

LSC · 74411-76483 · 2073 bp

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Pi 0.00106
MarkerSeek score 53.30
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2073
variable_sites
4
pi
0.00106
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 TCGATCTTAACCTGGTGATT AGGATAAACAGAAGGAAAATCCA 40.0 34.8 53.8 55.5 2299-2352 bp 1.0 40.4
clpP_p2 TCGATCTTAACCTGGTGATT ACATTACGTTTCCAAATCAAAGT 40.0 30.4 53.8 55.3 2249-2302 bp 1.0 40.4
clpP_p3 TCGATCTTAACCTGGTGATT AGGATAAACAGAAGGAAAATCCAA 40.0 33.3 53.8 56.3 2299-2352 bp 1.0 40.4
clpP_p4 TCGATCTTAACCTGGTGATT GGATAAACAGAAGGAAAATCCA 40.0 36.4 53.8 54.1 2298-2351 bp 1.0 40.4
clpP_p5 TCGATCTTAACCTGGTGATT AGGATAAACAGAAGGAAAATCCAAA 40.0 32.0 53.8 56.9 2299-2352 bp 1.0 40.4