Hotspot

ndhG-ndhI

SSC · 123827-124483 · 657 bp

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Pi 0.00671
MarkerSeek score 46.00
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
657
variable_sites
10
pi
0.00671
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TCCCAATGAAAAGGCAGAAA CTTTCAATTCAAAAACAATTACCA 40.0 25.0 55.4 53.2 806-836 bp 1.0 43.3
ndhG-ndhI_p2 TCCCAATGAAAAGGCAGAAAA CTTTCAATTCAAAAACAATTACCA 38.1 25.0 56.2 53.2 806-836 bp 1.0 43.3
ndhG-ndhI_p3 TCCCAATGAAAAGGCAGAAA TCTTTCAATTCAAAAACAATTACC 40.0 25.0 55.4 53.0 807-837 bp 1.0 43.3
ndhG-ndhI_p4 TCCCAATGAAAAGGCAGAAAA TCTTTCAATTCAAAAACAATTACC 38.1 25.0 56.2 53.0 807-837 bp 1.0 43.3
ndhG-ndhI_p5 TCCCAATGAAAAGGCAGAAAAA CTTTCAATTCAAAAACAATTACCA 36.4 25.0 57.0 53.2 806-836 bp 1.0 43.3