Hotspot

ccsA-ndhD

SSC · 120176-120437 · 262 bp

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Pi 0.0142
MarkerSeek score 38.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
262
variable_sites
13
pi
0.0142
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATCCCAGTGGTTGATGATGC TGGAATTTTTACCCCACGCA 50.0 45.0 57.9 58.0 1537-1538 bp 1.0 80.6
ccsA-ndhD_p2 ATCCCAGTGGTTGATGATGC TGCATTACCGGGAATGAGTG 50.0 50.0 57.9 58.0 1176-1177 bp 1.0 80.6
ccsA-ndhD_p3 ATCCCAGTGGTTGATGATGC TTAGGGGGTACAAGCTGTGA 50.0 50.0 57.9 58.0 1292-1293 bp 1.0 80.5
ccsA-ndhD_p4 TTTGCAATCCCAGTGGTTGA TGGAATTTTTACCCCACGCA 45.0 45.0 57.9 58.0 1543-1544 bp 1.0 80.4
ccsA-ndhD_p5 GCGCCGAGTGCTATTTTTAC TGGAATTTTTACCCCACGCA 50.0 45.0 57.9 58.0 1626-1627 bp 1.0 80.3