Hotspot

rpl32-trnL-UAG

SSC · 118615-119000 · 386 bp

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Pi 0.0104
MarkerSeek score 47.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
386
variable_sites
8
pi
0.0104
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAATCGGTTTCAACGGGGAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 472-477 bp 1.0 81.2
rpl32-trnL-UAG_p2 AAATCGGTTTCAACGGGGAA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 473-478 bp 1.0 81.1
rpl32-trnL-UAG_p3 AAATCGGTTTCAACGGGGAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.0 57.9 482-487 bp 1.0 80.2
rpl32-trnL-UAG_p4 AAATCGGTTTCAACGGGGAA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.0 57.8 481-486 bp 1.0 79.6
rpl32-trnL-UAG_p5 AAATCGGTTTCAACGGGGAA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.0 57.8 480-485 bp 1.0 79.6