Hotspot

trnN-GUU-ndhF

IRb · 114058-115009 · 952 bp

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Pi 0.0355
MarkerSeek score 80.70
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
952
variable_sites
69
pi
0.0355
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 AGGATTCGAACCTACGACCA TATTGGAGGTGGGCGAATCT 50.0 50.0 58.2 58.5 1105-1577 bp 1.0 82.0
trnN-GUU-ndhF_p2 AGGATTCGAACCTACGACCA GTGGGCGAATCTCGTCTTAT 50.0 50.0 58.2 57.5 1097-1569 bp 1.0 81.9
trnN-GUU-ndhF_p3 AGGATTCGAACCTACGACCA ATATTGGAGGTGGGCGAATC 50.0 50.0 58.2 57.4 1106-1578 bp 1.0 81.4
trnN-GUU-ndhF_p4 CCCCAAGTAGGATTCGAACC TATTGGAGGTGGGCGAATCT 55.0 50.0 57.7 58.5 1113-1585 bp 1.0 80.6
trnN-GUU-ndhF_p5 CCCCAAGTAGGATTCGAACC GTGGGCGAATCTCGTCTTAT 55.0 50.0 57.7 57.5 1105-1577 bp 1.0 80.5