Hotspot

psbE-petL

LSC · 68987-70211 · 1225 bp

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Pi 0.00503
MarkerSeek score 46.40
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1225
variable_sites
14
pi
0.00503
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ACTGACGAATAACCAACCCG AAGCAGCCAGTAGAAAACCA 50.0 45.0 57.9 57.3 1379-1392 bp 1.0 75.2
psbE-petL_p2 ACTGACGAATAACCAACCCG AGCAGCCAGTAGAAAACCAA 50.0 45.0 57.9 57.3 1378-1391 bp 1.0 75.2
psbE-petL_p3 AATAACCAACCCGCGATGAA AAGCAGCCAGTAGAAAACCA 45.0 45.0 57.9 57.3 1372-1385 bp 1.0 75.0
psbE-petL_p4 AATAACCAACCCGCGATGAA AGCAGCCAGTAGAAAACCAA 45.0 45.0 57.9 57.3 1371-1384 bp 1.0 75.0
psbE-petL_p5 AACCAACCCGCGATGAATAG AGCAGCCAGTAGAAAACCAA 50.0 45.0 58.3 57.3 1368-1381 bp 1.0 73.2