Hotspot

accD-psaI

LSC · 63931-64831 · 901 bp

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Pi 0.00403
MarkerSeek score 53.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
901
variable_sites
10
pi
0.00403
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 ATTTCAGCTCCATGGGTTCC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.9 58.0 1015-1033 bp 1.0 79.8
accD-psaI_p2 ATTTCAGCTCCATGGGTTCC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.9 57.9 1021-1039 bp 1.0 79.1
accD-psaI_p3 ATTTCAGCTCCATGGGTTCC GCCGGAAATACTAGGCCTAC 50.0 55.0 57.9 57.5 1004-1022 bp 1.0 76.4
accD-psaI_p4 CCATGGGTTCCTTCCTTTGA CCATTGCAATTGCCGGAAAT 50.0 45.0 57.4 58.0 1006-1024 bp 1.0 76.1
accD-psaI_p5 TCCATGGGTTCCTTCCTTTG CCATTGCAATTGCCGGAAAT 50.0 45.0 57.4 58.0 1007-1025 bp 1.0 76.1