Hotspot

trnE-UUC-trnT-GGU

LSC · 34231-34901 · 671 bp

Back to Alisma

Pi 0.00596
MarkerSeek score 45.20
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
671
variable_sites
9
pi
0.00596
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTAGTATCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.1 58.0 809-810 bp 1.0 80.3
trnE-UUC-trnT-GGU_p2 CGTAGTATCCTACCCCCAGG CCATGGCGTTACTCTACCAC 60.0 55.0 58.1 58.1 788-789 bp 1.0 79.9
trnE-UUC-trnT-GGU_p3 CGTAGTATCCTACCCCCAGG TGGCGTTACTCTACCACTGA 60.0 50.0 58.1 58.1 785-786 bp 1.0 79.7
trnE-UUC-trnT-GGU_p4 CGTAGTATCCTACCCCCAGG GACGTGGAAAAAGCCCCTTA 60.0 50.0 58.1 58.1 837-838 bp 1.0 79.5
trnE-UUC-trnT-GGU_p5 CGTAGTATCCTACCCCCAGG TAAGACGTGGAAAAAGCCCC 60.0 50.0 58.1 58.1 840-841 bp 1.0 79.5