Hotspot

atpH-atpI

LSC · 15750-16742 · 993 bp

Pi 0.00364

MarkerSeek score 50.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 9
pi: 0.00364
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.0	57.9	1082-1085 bp	1.0	79.7
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.9	57.9	1138-1141 bp	1.0	79.1
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.2	57.9	1083-1086 bp	1.0	78.6
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	ATTTTTGCAACCTTAGCCGC	55.0	45.0	58.2	57.9	1142-1145 bp	1.0	78.5
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.3	57.9	1092-1095 bp	1.0	77.9