Hotspot

atpF-atpH

LSC · 14931-15503 · 573 bp

Back to Alisma

Pi 0.00133
MarkerSeek score 54.70
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
573
variable_sites
2
pi
0.00133
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 AATGGCCCAAGGAAACGAAA TTTACGGATTGGTCGTAGCA 45.0 45.0 58.3 57.0 664-674 bp 1.0 70.2
atpF-atpH_p2 AATGGCCCAAGGAAACGAAA TGGTCGTAGCATTAGCACTT 45.0 45.0 58.3 56.9 655-665 bp 1.0 70.1
atpF-atpH_p3 AATGGCCCAAGGAAACGAAA TTGGTCGTAGCATTAGCACT 45.0 45.0 58.3 56.9 656-666 bp 1.0 70.1
atpF-atpH_p4 AATGGCCCAAGGAAACGAAA ACGGATTGGTCGTAGCATTAG 45.0 47.6 58.3 57.9 661-671 bp 1.0 69.8
atpF-atpH_p5 AATGGCCCAAGGAAACGAAA GGATTGGTCGTAGCATTAGCA 45.0 47.6 58.3 58.2 659-669 bp 1.0 69.2