Hotspot

trnS-GCU-psbI

LSC · 7645-7871 · 227 bp

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Pi 0.0204
MarkerSeek score 47.50
Primer available yes
Label trnS-GCU-psbI

Feature Metadata

label_name
trnS-GCU-psbI
feature_type
igs
region
LSC
length_bp
227
variable_sites
9
pi
0.0204
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psbI_p1 CACAGGCCTCGGTCATTTTA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1113-1147 bp 1.0 82.5
trnS-GCU-psbI_p2 CACAGGCCTCGGTCATTTTA CGTCCCAGGTTGATTCCAAT 50.0 50.0 57.9 57.9 1097-1131 bp 1.0 82.1
trnS-GCU-psbI_p3 AGCTTACCTTGCTTTGCTGA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.7 58.1 842-873 bp 1.0 81.7
trnS-GCU-psbI_p4 CACAGGCCTCGGTCATTTTA CTTCCGTCCCAGGTTGATTC 50.0 55.0 57.9 58.3 1101-1135 bp 1.0 81.1
trnS-GCU-psbI_p5 CGGATCCATTACACCCAACC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1195-1229 bp 1.0 80.5