Hotspot

rps16-trnS-GCU

LSC · 6556-7572 · 1017 bp

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Pi 0.00507
MarkerSeek score 51.10
Primer available yes
Label rps16-trnS-GCU

Feature Metadata

label_name
rps16-trnS-GCU
feature_type
igs
region
LSC
length_bp
1017
variable_sites
13
pi
0.00507
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnS-GCU_p1 AAGTCGCACGTTGCTTTCTA AGTACTAATTTCATCATGGGTCT 45.0 34.8 58.1 54.8 1086-1089 bp 1.0 41.2
rps16-trnS-GCU_p2 TGATGTCCTTCAAGTCGCAC AGTACTAATTTCATCATGGGTCT 50.0 34.8 58.2 54.8 1097-1100 bp 1.0 41.2
rps16-trnS-GCU_p3 AAGTCGCACGTTGCTTTCTA AGTACTAATTTCATCATGGGTC 45.0 36.4 58.1 53.3 1086-1089 bp 1.0 41.2
rps16-trnS-GCU_p4 AAGTCGCACGTTGCTTTCTA GTACTAATTTCATCATGGGTCT 45.0 36.4 58.1 53.3 1085-1088 bp 1.0 41.2
rps16-trnS-GCU_p5 TGATGTCCTTCAAGTCGCAC GTACTAATTTCATCATGGGTCT 50.0 36.4 58.2 53.3 1096-1099 bp 1.0 41.2