Hotspot

rpoB

LSC · 12817-15999 · 3183 bp

Back to Alfredia

Pi 0.000943

MarkerSeek score 55.90

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3183
variable_sites: 3
pi: 0.000943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GCAAGGATATGGGTTCGTGT	ATTTCGTCGACCAATCCCTC	50.0	50.0	58.0	57.7	294 bp	1.0	77.7
rpoB_p2	GCAAGGATATGGGTTCGTGT	CGACCAATCCCTCCCAATTC	50.0	55.0	58.0	58.3	287 bp	1.0	77.7
rpoB_p3	GTTCGTGTGAGTAGGCAACA	ATTTCGTCGACCAATCCCTC	50.0	50.0	58.1	57.7	282 bp	1.0	77.0
rpoB_p4	GTTCGTGTGAGTAGGCAACA	CGACCAATCCCTCCCAATTC	50.0	55.0	58.1	58.3	275 bp	1.0	76.9
rpoB_p5	GCAAGGATATGGGTTCGTGT	CCAATCCCTCCCAATTCACA	50.0	50.0	58.0	57.5	284 bp	1.0	76.0