Hotspot

matK

LSC · 2067-3587 · 1521 bp

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Pi 0.00197

MarkerSeek score 59.80

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	GTGATCAAATTTTTATTTACAACG	ACCCCCAAAATCCTCTACCT	25.0	50.0	52.1	57.6	1598 bp	1.0	40.2
matK_p2	GTGATCAAATTTTTATTTACAACG	AACCCCCAAAATCCTCTACC	25.0	50.0	52.1	56.8	1599 bp	1.0	40.2
matK_p3	GTGATCAAATTTTTATTTACAACG	AACCCCCAAAATCCTCTACCT	25.0	47.6	52.1	58.3	1599 bp	1.0	40.2
matK_p4	GTGATCAAATTTTTATTTACAACG	ACCCCCAAAATCCTCTACCTT	25.0	47.6	52.1	58.3	1598 bp	1.0	40.2
matK_p5	GTGATCAAATTTTTATTTACAACG	CCCCCAAAATCCTCTACCTT	25.0	50.0	52.1	56.5	1597 bp	1.0	40.2