Hotspot

ndhF-trnN-GUU

IRa · 127968-128873 · 906 bp

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Pi 0.00221
MarkerSeek score 60.00
Primer available yes
Label ndhF-trnN-GUU

Feature Metadata

label_name
ndhF-trnN-GUU
feature_type
igs
region
IRa
length_bp
906
variable_sites
2
pi
0.00221
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnN-GUU_p1 GGGTGGGCGTATTTCTTCTT AGGATTCGAACCTACGACCA 50.0 50.0 57.9 58.2 1053 bp 1.0 77.9
ndhF-trnN-GUU_p2 GGGTGGGCGTATTTCTTCTT CGAGGTGTTGACGGTGATTT 50.0 50.0 57.9 58.5 1089 bp 1.0 75.2
ndhF-trnN-GUU_p3 GGGTGGGCGTATTTCTTCTT GGATTCGAACCTACGACCAA 50.0 50.0 57.9 57.3 1052 bp 1.0 73.9
ndhF-trnN-GUU_p4 GGGTGGGCGTATTTCTTCTT GAGTATCCCGGACCTACACC 50.0 60.0 57.9 58.7 1108 bp 1.0 73.8
ndhF-trnN-GUU_p5 TTAAATCGGTAGGGGGTGGG AGGATTCGAACCTACGACCA 55.0 50.0 58.8 58.2 1066 bp 1.0 72.6