Hotspot

atpI-atpH

LSC · 25076-26212 · 1137 bp

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Pi 0.00088

MarkerSeek score 59.30

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1137
variable_sites: 1
pi: 0.00088
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	50.0	50.0	58.3	57.9	1329-1330 bp	1.0	77.1
atpI-atpH_p2	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	50.0	55.0	58.3	58.2	1333-1334 bp	1.0	76.5
atpI-atpH_p3	TTTTTGCAACTTTAGCCGCG	TACCTTGACCAACTCCAGGT	45.0	50.0	58.5	57.9	1281-1282 bp	1.0	75.0
atpI-atpH_p4	TCCCTGTCATGTTCCTTGGA	AGGTCCAATAGAAGCAAGCC	50.0	50.0	58.3	57.6	1313-1314 bp	1.0	74.6
atpI-atpH_p5	TCCCTGTCATGTTCCTTGGA	GTCCAATAGAAGCAAGCCCT	50.0	50.0	58.3	57.6	1311-1312 bp	1.0	74.6