Hotspot

rps16-trnQ-UUG

LSC · 6264-7198 · 935 bp

Back to Alfredia

Pi 0.00107
MarkerSeek score 59.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
935
variable_sites
1
pi
0.00107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1008-1045 bp 1.0 78.4
rps16-trnQ-UUG_p2 TGCTTTCTACCGCATCGTTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 997-1034 bp 1.0 77.9
rps16-trnQ-UUG_p3 TTGCTTTCTACCGCATCGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.2 58.1 998-1035 bp 1.0 77.9
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1023-1060 bp 1.0 76.5
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1022-1059 bp 1.0 76.5