Hotspot

ycf1

SSC · 123316-128940 · 5625 bp

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Pi 0.0116

MarkerSeek score 46.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 241
pi: 0.0116
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCCATCACTTGGGCATGT	AGCCCAGTTTACGAAGATTCT	45.0	42.9	57.7	57.0	245 bp	1.0	66.9
ycf1_p2	TTTCCATCACTTGGGCATGT	AGCCCAGTTTACGAAGATTCTT	45.0	40.9	57.7	57.7	245 bp	1.0	64.5
ycf1_p3	GGTTTTCCATCACTTGGGCA	AGCCCAGTTTACGAAGATTCT	50.0	42.9	58.7	57.0	248 bp	1.0	64.3
ycf1_p4	TTTCCATCACTTGGGCATGT	AGCCCAGTTTACGAAGATTC	45.0	45.0	57.7	55.5	245 bp	1.0	62.5
ycf1_p5	TTTCCATCACTTGGGCATGT	GCCCAGTTTACGAAGATTCT	45.0	45.0	57.7	55.5	244 bp	1.0	62.5