Hotspot

ndhF-rpl32

SSC · 111875-112774 · 900 bp

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Pi 0.0279
MarkerSeek score 72.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
900
variable_sites
80
pi
0.0279
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGAACAAGCAAAAGTCCAAA AAGCTTTTACTGCAGCTAGA 38.1 40.0 56.8 54.7 820-1095 bp 1.0 50.8
ndhF-rpl32_p2 TGGAACAAGCAAAAGTCCAA AAGCTTTTACTGCAGCTAGA 40.0 40.0 56.0 54.7 820-1095 bp 0.923077 45.8
ndhF-rpl32_p3 TGGAACAAGCAAAAGTCCAA AAAGCTTTTACTGCAGCTAGA 40.0 38.1 56.0 55.5 821-1096 bp 0.923077 45.8
ndhF-rpl32_p4 TGGAACAAGCAAAAGTCCAA AGCTTTTACTGCAGCTAGAT 40.0 40.0 56.0 54.4 819-1094 bp 0.923077 45.8
ndhF-rpl32_p5 TGGAACAAGCAAAAGTCCAA AAGCTTTTACTGCAGCTAGAT 40.0 38.1 56.0 55.3 820-1095 bp 0.923077 45.8