Hotspot

trnT-GGU-psbD

LSC · 31444-32373 · 930 bp

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Pi 0.0161
MarkerSeek score 61.10
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
930
variable_sites
57
pi
0.0161
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.95
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AGCAATAGACCAGACCAACC 55.0 50.0 58.1 57.2 973-1200 bp 1.0 81.4
trnT-GGU-psbD_p2 GTGGTAGAGTAACGCCATGG GCAATAGACCAGACCAACCT 55.0 50.0 58.1 57.2 972-1199 bp 1.0 81.4
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA AGCAATAGACCAGACCAACC 50.0 50.0 58.1 57.2 976-1203 bp 1.0 81.2
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GCAATAGACCAGACCAACCT 50.0 50.0 58.1 57.2 975-1202 bp 1.0 81.2
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GCAATAGACCAGACCAACCT 50.0 50.0 58.3 57.2 949-1176 bp 1.0 79.7