Hotspot

petN-psbM

LSC · 28095-29146 · 1052 bp

Back to Aletris

Pi 0.0162

MarkerSeek score 64.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1052
variable_sites: 59
pi: 0.0162
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	ATTGCTACTGCGCTGTTCAT	45.0	45.0	58.1	58.3	1178-1269 bp	1.0	82.2
petN-psbM_p2	CCCTTTCGCTCGTAGTATGG	ATTGCTACTGCGCTGTTCAT	55.0	45.0	57.9	58.3	1171-1262 bp	1.0	82.0
petN-psbM_p3	CCTTTCGCTCGTAGTATGGG	ATTGCTACTGCGCTGTTCAT	55.0	45.0	57.9	58.3	1170-1261 bp	1.0	82.0
petN-psbM_p4	CTTTCGCTCGTAGTATGGGG	ATTGCTACTGCGCTGTTCAT	55.0	45.0	57.9	58.3	1169-1260 bp	1.0	82.0
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1216-1307 bp	1.0	81.7