Hotspot

rps2-rpoC2

LSC · 15690-15946 · 257 bp

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Pi 0.0499

MarkerSeek score 70.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 257
variable_sites: 29
pi: 0.0499
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GCTTCCATCATCTCTGGCAA	TGCATCCCAAATAATTCTCATGG	50.0	39.1	58.0	57.2	340-351 bp	1.0	57.7
rps2-rpoC2_p2	CCTGCTTCCATCATCTCTGG	TGCATCCCAAATAATTCTCATGG	55.0	39.1	57.7	57.2	343-354 bp	1.0	55.9
rps2-rpoC2_p3	GCAGAAATGTAAGGGGCCAT	TGCATCCCAAATAATTCTCATGG	50.0	39.1	58.2	57.2	403-414 bp	1.0	55.2
rps2-rpoC2_p4	CTGCTTCCATCATCTCTGGC	TGCATCCCAAATAATTCTCATGG	55.0	39.1	58.4	57.2	342-353 bp	1.0	54.8
rps2-rpoC2_p5	GCTTCCATCATCTCTGGCAA	TTGCATCCCAAATAATTCTCATGG	50.0	37.5	58.0	57.8	341-352 bp	1.0	54.7