Hotspot

trnS-GCU-trnG-UCC

LSC · 7575-8425 · 851 bp

Back to Aletris

Pi 0.0192

MarkerSeek score 69.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 74
pi: 0.0192
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TACCACTAAACTATGCCCGC	45.0	50.0	58.0	57.4	879-938 bp	1.0	85.3
trnS-GCU-trnG-UCC_p2	ATAAGGAGCGGAGAGAGAGG	TACCACTAAACTATGCCCGC	55.0	50.0	57.7	57.4	935-994 bp	1.0	83.1
trnS-GCU-trnG-UCC_p3	CGGAGAGAGAGGGATTCGAA	TACCACTAAACTATGCCCGC	55.0	50.0	58.3	57.4	927-986 bp	1.0	82.8
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	TACCACTAAACTATGCCCGC	50.0	50.0	57.6	57.4	865-924 bp	1.0	82.5
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	TACCACTAAACTATGCCCGC	55.0	50.0	58.5	57.4	860-919 bp	1.0	81.7