Hotspot

psbI-trnS-GCU

LSC · 7385-7486 · 102 bp

Back to Aletris

Pi 0.0302

MarkerSeek score 66.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 102
variable_sites: 9
pi: 0.0302
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCTGGGCGTGAAGAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.6	58.0	192-199 bp	1.0	82.7
psbI-trnS-GCU_p2	GTAATCCTGGGCGTGAAGAA	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.6	58.2	147-154 bp	1.0	82.5
psbI-trnS-GCU_p3	GTAATCCTGGGCGTGAAGAA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	57.6	58.2	160-167 bp	1.0	82.4
psbI-trnS-GCU_p4	GTAATCCTGGGCGTGAAGAA	GGAATTGGGAGAGATGGCTG	50.0	55.0	57.6	58.0	219-226 bp	1.0	82.0
psbI-trnS-GCU_p5	GTAATCCTGGGCGTGAAGAA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.6	58.1	216-223 bp	1.0	81.4