Hotspot

trnQ-UUG-psbK

LSC · 6286-6640 · 355 bp

Back to Aletris

Pi 0.0238

MarkerSeek score 60.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 34
pi: 0.0238
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CTGCCTTACCACTTGGCC	GGGCAATTTGGCGAAGAAAA	61.1	45.0	58.0	57.8	461-483 bp	1.0	70.2
trnQ-UUG-psbK_p2	CCTTACCACTTGGCCACG	GGGCAATTTGGCGAAGAAAA	61.1	45.0	58.0	57.8	458-480 bp	1.0	70.1
trnQ-UUG-psbK_p3	AAAACCCGCTGCCTTACC	GGGCAATTTGGCGAAGAAAA	55.6	45.0	57.9	57.8	469-491 bp	1.0	69.4
trnQ-UUG-psbK_p4	CCAAAACCCGCTGCCTTA	GGGCAATTTGGCGAAGAAAA	55.6	45.0	57.9	57.8	471-493 bp	1.0	69.3
trnQ-UUG-psbK_p5	CTGCCTTACCACTTGGCC	GGCATAAGACTCGGGCAATT	61.1	50.0	58.0	58.3	473-495 bp	1.0	68.9