Hotspot

psbA-trnK-UUU

LSC · 1063-1284 · 222 bp

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Pi 0.0274

MarkerSeek score 69.60

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 222
variable_sites: 17
pi: 0.0274
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TAGGCTTGTACTTTCGCGTC	AAAATGCAAGCACGGTTTGG	50.0	45.0	58.0	58.4	358-367 bp	1.0	82.0
psbA-trnK-UUU_p2	TAGGCTTGTACTTTCGCGTC	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.0	58.6	297-306 bp	1.0	81.6
psbA-trnK-UUU_p3	TAGGCTTGTACTTTCGCGTC	TGAAAAATGCAAGCACGGTT	50.0	40.0	58.0	57.4	361-370 bp	1.0	80.6
psbA-trnK-UUU_p4	TAGGCTTGTACTTTCGCGTC	AAAGCCGTGTGCAATGAAAA	50.0	40.0	58.0	57.4	375-384 bp	1.0	80.4
psbA-trnK-UUU_p5	TAGGCTTGTACTTTCGCGTC	AAGCCGTGTGCAATGAAAAA	50.0	40.0	58.0	57.4	374-383 bp	1.0	80.4