Hotspot

psaC

SSC · 119272-119517 · 246 bp

Back to Aldama

Pi 0
MarkerSeek score 20.90
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
20.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 TCACGTTCAAAAATGGATAGA TTTTTGGGCACAGGTTTTGG 33.3 45.0 53.2 57.6 325-527 bp 1.0 40.2
psaC_p2 TCACGTTCAAAAATGGATAGATC TTTTTGGGCACAGGTTTTGG 34.8 45.0 55.1 57.6 325-337 bp 1.0 40.2
psaC_p3 ATCACGTTCAAAAATGGATAGA TTTTTGGGCACAGGTTTTGG 31.8 45.0 53.8 57.6 326-528 bp 1.0 40.2
psaC_p4 TCACGTTCAAAAATGGATAGAT TTTTTGGGCACAGGTTTTGG 31.8 45.0 53.8 57.6 325-337 bp 1.0 40.2
psaC_p5 TCACGTTCAAAAATGGATAGA CACAGGTTTTGGCGGTAAAA 33.3 45.0 53.2 57.1 317-519 bp 1.0 40.2