Hotspot

clpP

LSC · 69525-71506 · 1982 bp

Pi 0.00205

MarkerSeek score 47.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1982
variable_sites: 27
pi: 0.00205
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	TTCTATGAGCGAATGTGCCC	50.0	50.0	58.0	58.0	2489-2530 bp	1.0	82.3
clpP_p2	GATCTGAACGAGTCGCACAT	TTCTATGAGCGAATGTGCCC	50.0	50.0	58.1	58.0	2492-2533 bp	1.0	81.7
clpP_p3	CATGATCTGAACGAGTCGCA	TTCTATGAGCGAATGTGCCC	50.0	50.0	58.1	58.0	2495-2536 bp	1.0	81.7
clpP_p4	CTGAACGAGTCGCACATACA	CTATGAGCGAATGTGCCCAT	50.0	50.0	58.0	58.1	2487-2528 bp	1.0	81.7
clpP_p5	CGGCAGGGTGAATTTCAGAA	GGCTTTGTTACGTTTCCACAT	50.0	42.9	58.5	57.4	2067-2095 bp	1.0	66.1