Hotspot

ccsA

SSC · 121397-122365 · 969 bp

Back to Aldama

Pi 0.000914
MarkerSeek score 42.80
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
969
variable_sites
11
pi
0.000914
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GAGAACCGCGTGAATCACTA GCGGCATGCCATCTTCTAAA 50.0 50.0 57.7 59.0 1199-1219 bp 0.935484 69.4
ccsA_p2 GCCGGTTGAGACAAGATGTT GCGGCATGCCATCTTCTAAA 50.0 50.0 58.5 59.0 1155-1175 bp 0.967742 69.0
ccsA_p3 GAGAACCGCGTGAATCACTA GCGGCATGCCATCTTCTAA 50.0 52.6 57.7 58.3 1199-1219 bp 0.935484 66.8
ccsA_p4 GCCGGTTGAGACAAGATGTT GCGGCATGCCATCTTCTAA 50.0 52.6 58.5 58.3 1155-1175 bp 0.967742 66.4
ccsA_p5 GAGAACCGCGTGAATCACTA CGGCATGCCATCTTCTAAAAG 50.0 47.6 57.7 57.7 1198-1218 bp 0.935484 66.4