Hotspot

ndhI-ndhG

SSC · 117600-117985 · 386 bp

Back to Aldama

Pi 0.0103
MarkerSeek score 56.80
Primer available yes
Label ndhI-ndhG

Feature Metadata

label_name
ndhI-ndhG
feature_type
igs
region
SSC
length_bp
386
variable_sites
21
pi
0.0103
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.98
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhG_p1 TCAAATTGCTTTGGGTCGGT TACTACTCCCAGACCCCCTA 45.0 55.0 58.3 57.7 551-572 bp 1.0 78.9
ndhI-ndhG_p2 TCAAATTGCTTTGGGTCGGT ATACTACTCCCAGACCCCCT 45.0 55.0 58.3 58.4 552-573 bp 1.0 78.4
ndhI-ndhG_p3 TCAAATTGCTTTGGGTCGGT AATACTACTCCCAGACCCCC 45.0 55.0 58.3 57.5 553-574 bp 1.0 77.4
ndhI-ndhG_p4 CAAATTGCTTTGGGTCGGTT TACTACTCCCAGACCCCCTA 45.0 55.0 57.5 57.7 550-571 bp 1.0 77.0
ndhI-ndhG_p5 CAAATTGCTTTGGGTCGGTT ATACTACTCCCAGACCCCCT 45.0 55.0 57.5 58.4 551-572 bp 1.0 76.5