Hotspot

ycf4-cemA

LSC · 59917-60754 · 838 bp

Back to Aldama

Pi 0.00357
MarkerSeek score 56.70
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
838
variable_sites
26
pi
0.00357
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGAGAATTTTACTCCGCGGG GAGAAATCCACCAGGGCAAA 50.0 50.0 58.0 58.1 989-2623 bp 1.0 82.1
ycf4-cemA_p2 GAGCCATTCCATTGACTCGT GAGAAATCCACCAGGGCAAA 50.0 50.0 58.0 58.1 1014-2262 bp 1.0 81.9
ycf4-cemA_p3 TTGGCCTATTTCTTGCGTGT GAGAAATCCACCAGGGCAAA 45.0 50.0 58.1 58.1 946-2643 bp 1.0 81.3
ycf4-cemA_p4 AAAAAGCTGCTGAATTGGCC GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 960-2637 bp 1.0 80.6
ycf4-cemA_p5 AAAGCTGCTGAATTGGCCTA GAGAAATCCACCAGGGCAAA 45.0 50.0 57.8 58.1 958-2635 bp 1.0 80.3