Hotspot

ndhC-trnV-UAC

LSC · 49951-50888 · 938 bp

Back to Aldama

Pi 0.00372
MarkerSeek score 63.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
938
variable_sites
33
pi
0.00372
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TTCTCCGGTCCTTTGCTACT GCATGTTGGGTCTTTGAAGC 50.0 50.0 58.4 57.9 928-1173 bp 1.0 80.9
ndhC-trnV-UAC_p2 CTTTGCTACTCGGGGCTAAA GCATGTTGGGTCTTTGAAGC 50.0 50.0 57.6 57.9 918-1163 bp 1.0 80.7
ndhC-trnV-UAC_p3 TTCTCCGGTCCTTTGCTACT AGTCCGTATAGCCCTAGAGC 50.0 55.0 58.4 57.8 928-1092 bp 1.0 80.2
ndhC-trnV-UAC_p4 TTCTCCGGTCCTTTGCTACT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 927-1109 bp 1.0 80.1
ndhC-trnV-UAC_p5 TTTCTCCGGTCCTTTGCTAC GCATGTTGGGTCTTTGAAGC 50.0 50.0 57.5 57.9 929-1174 bp 1.0 80.0