Hotspot

psbC-trnS-UGA

LSC · 34974-35221 · 248 bp

Back to Aldama

Pi 0.00872
MarkerSeek score 49.70
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
248
variable_sites
7
pi
0.00872
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.95
markerseek_score
49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 358-366 bp 1.0 81.9
psbC-trnS-UGA_p2 TCTTCGTAGGGCATTTGTGG GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.9 58.0 441-449 bp 1.0 81.7
psbC-trnS-UGA_p3 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 366-374 bp 1.0 81.4
psbC-trnS-UGA_p4 TCTTCGTAGGGCATTTGTGG GTCTTTCGGAGAGATGGCTG 50.0 55.0 57.9 58.1 449-457 bp 1.0 81.3
psbC-trnS-UGA_p5 TCTTCGTAGGGCATTTGTGG TCTTTCGGAGAGATGGCTGA 50.0 50.0 57.9 58.1 448-456 bp 1.0 80.6