Hotspot

trnT-GGU-psbD

LSC · 31355-32542 · 1188 bp

Back to Aldama

Pi 0.00151
MarkerSeek score 57.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1188
variable_sites
14
pi
0.00151
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 ATGGTAACGCGTAAACCCTC GAAACGGTCCCTACGTAACC 50.0 55.0 58.0 58.0 1305-1319 bp 1.0 82.8
trnT-GGU-psbD_p2 GTAATCCCATGGTAACGCGT GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1313-1327 bp 1.0 82.5
trnT-GGU-psbD_p3 ATGGTAACGCGTAAACCCTC GGACCAGCCTACAAAAACGA 50.0 50.0 58.0 58.1 1323-1337 bp 1.0 82.0
trnT-GGU-psbD_p4 GTAATCCCATGGTAACGCGT GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1331-1345 bp 1.0 81.6
trnT-GGU-psbD_p5 CGACCCGTTCAGGAATTCAA GAAACGGTCCCTACGTAACC 50.0 55.0 58.2 58.0 1366-1380 bp 1.0 81.2