Hotspot

atpI-atpH

LSC · 25338-26494 · 1157 bp

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Pi 0.00143
MarkerSeek score 57.80
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1157
variable_sites
19
pi
0.00143
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 CCCGTCATGTTCCTTGGATT ATAACGGAAGCGGCAGAAAT 50.0 45.0 57.9 58.0 1285-1297 bp 1.0 81.6
atpI-atpH_p2 CCCGTCATGTTCCTTGGATT TACCTTGACCAACTCCAGGT 50.0 50.0 57.9 57.9 1341-1353 bp 1.0 80.9
atpI-atpH_p3 CCCGTCATGTTCCTTGGATT AGCCAATCCAGCAGCAATAA 50.0 45.0 57.9 57.9 1301-1313 bp 1.0 80.7
atpI-atpH_p4 CCCGTCATGTTCCTTGGATT AATAACGGAAGCGGCAGAAA 50.0 45.0 57.9 58.2 1286-1298 bp 1.0 80.5
atpI-atpH_p5 CCCGTCATGTTCCTTGGATT GCAGTACCTTGACCAACTCC 50.0 55.0 57.9 58.2 1345-1357 bp 1.0 80.3