Hotspot

rps16-trnQ-UUG

LSC · 6336-7313 · 978 bp

Back to Aldama

Pi 0.00289
MarkerSeek score 56.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
978
variable_sites
36
pi
0.00289
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.98
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1046-1070 bp 1.0 81.5
rps16-trnQ-UUG_p2 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1061-1085 bp 1.0 79.7
rps16-trnQ-UUG_p3 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1060-1084 bp 1.0 79.7
rps16-trnQ-UUG_p4 CCAAGAGCACCTTCATTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 1117-1141 bp 1.0 78.5
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1050-1074 bp 1.0 76.8