Hotspot

trnK-UUU-rps16

LSC · 4365-5214 · 850 bp

Back to Aldama

Pi 0.0017
MarkerSeek score 61.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
850
variable_sites
16
pi
0.0017
unique_haplotype_count
27
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 982-992 bp 1.0 79.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 981-991 bp 1.0 79.1
trnK-UUU-rps16_p3 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1067-1077 bp 1.0 78.4
trnK-UUU-rps16_p4 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1044-1053 bp 0.967742 78.2
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 1043-1052 bp 0.967742 78.2