Hotspot

ndhF

SSC · 108534-110765 · 2232 bp

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Pi 0.0183

MarkerSeek score 52.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 120
pi: 0.0183
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCGCCCCTTACGGTATATA	TGCTGCCCAATACCCTTTTT	50.0	45.0	57.8	58.0	2950 bp	0.166667	45.3
ndhF_p2	AGCGCCCCTTACGGTATATA	AAAAGCTTTCAATGCTGCCC	50.0	45.0	57.8	57.8	2962 bp	0.166667	44.0
ndhF_p3	AGCGCCCCTTACGGTATATA	AATGCTGCCCAATACCCTTT	50.0	45.0	57.8	57.7	2952 bp	0.166667	43.4
ndhF_p4	AGCGCCCCTTACGGTATATA	ATGCTGCCCAATACCCTTTT	50.0	45.0	57.8	57.7	2951 bp	0.166667	43.4
ndhF_p5	AAGCGCCCCTTACGGTATAT	TGCTGCCCAATACCCTTTTT	50.0	45.0	58.7	58.0	2951 bp	0.166667	41.8