Hotspot

rpl32-trnL-UAG

SSC · 111532-112198 · 667 bp

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Pi 0.0435

MarkerSeek score 78.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 667
variable_sites: 87
pi: 0.0435
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAAAAGGGTATTGGGCAGCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	741-830 bp	1.0	91.8
rpl32-trnL-UAG_p2	AAAAAGGGTATTGGGCAGCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	742-831 bp	1.0	91.7
rpl32-trnL-UAG_p3	AAAAAGGGTATTGGGCAGCA	ATCCACACAAAAGAGCTGCA	45.0	45.0	58.0	58.0	1348-1437 bp	1.0	91.6
rpl32-trnL-UAG_p4	AAAAAGGGTATTGGGCAGCA	TCCACACAAAAGAGCTGCAT	45.0	45.0	58.0	58.0	1347-1436 bp	1.0	91.6
rpl32-trnL-UAG_p5	AAAAAGGGTATTGGGCAGCA	CCCGAAGTAGTGAAACCCTG	45.0	55.0	58.0	57.9	1235-1324 bp	1.0	91.1