Hotspot

ndhF-rpl32

SSC · 110766-111372 · 607 bp

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Pi 0.042

MarkerSeek score 80.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 607
variable_sites: 74
pi: 0.042
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAACGGT	AACTCGAGGTGCGCTTTTT	50.0	47.4	57.8	58.3	655-734 bp	1.0	80.1
ndhF-rpl32_p2	CTGGAAGTGGAATGAACGGT	GAACTCGAGGTGCGCTTTTT	50.0	50.0	57.8	59.4	656-735 bp	1.0	79.2
ndhF-rpl32_p3	AAAGTAGAAGCCCTGCTCCT	AACTCGAGGTGCGCTTTTT	50.0	47.4	58.3	58.3	688-767 bp	1.0	78.9
ndhF-rpl32_p4	AAAGTAGAAGCCCTGCTCCT	GAACTCGAGGTGCGCTTTTT	50.0	50.0	58.3	59.4	689-768 bp	1.0	78.0
ndhF-rpl32_p5	AAAAGTAGAAGCCCTGCTCC	AACTCGAGGTGCGCTTTTT	50.0	47.4	57.5	58.3	689-768 bp	1.0	77.6