Hotspot

petA-psbJ

LSC · 61210-62140 · 931 bp

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Pi 0.0357

MarkerSeek score 80.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 931
variable_sites: 94
pi: 0.0357
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AAGGTTCAGTTGGCCGAAAT	AGGTACTGTAGCCGGTATTGT	45.0	47.6	58.0	58.3	975-1101 bp	1.0	81.4
petA-psbJ_p2	AAGGTTCAGTTGGCCGAAAT	GGTACTGTAGCCGGTATTGT	45.0	50.0	58.0	56.7	974-1100 bp	1.0	81.4
petA-psbJ_p3	AAGGTTCAGTTGGCCGAAAT	ACTGTAGCCGGTATTGTTGT	45.0	45.0	58.0	56.9	993-1097 bp	0.916667	77.3
petA-psbJ_p4	TTGAGAAGGTTCAGTTGGCC	ACTGTAGCCGGTATTGTTGT	50.0	45.0	58.0	56.9	998-1102 bp	0.916667	77.2
petA-psbJ_p5	TTGAGAAGGTTCAGTTGGCC	AGGTACTGTAGCCGGTATTGT	50.0	47.6	58.0	58.3	1002-1106 bp	0.916667	76.2