Hotspot

ndhC-trnV-UAC

LSC · 49613-50191 · 579 bp

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Pi 0.0508

MarkerSeek score 83.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 73
pi: 0.0508
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CATATTCGTAAAGCAGCAGCA	GTTCGAGTCCGTATAGCCCT	42.9	55.0	57.0	58.7	565-622 bp	1.0	70.9
ndhC-trnV-UAC_p2	CATATTCGTAAAGCAGCAGCA	TTCGAGTCCGTATAGCCCTA	42.9	50.0	57.0	57.1	564-621 bp	1.0	68.9
ndhC-trnV-UAC_p3	TCATATTCGTAAAGCAGCAGC	GTTCGAGTCCGTATAGCCCT	42.9	55.0	56.8	58.7	566-623 bp	1.0	68.8
ndhC-trnV-UAC_p4	CATATTCGTAAAGCAGCAGCA	AGGTCTACGGTTCGAGTCC	42.9	57.9	57.0	58.1	574-631 bp	1.0	67.3
ndhC-trnV-UAC_p5	TATTCGTAAAGCAGCAGCAT	GTTCGAGTCCGTATAGCCCT	40.0	55.0	55.5	58.7	563-620 bp	1.0	66.8