Hotspot

ycf3-trnS-GGA

LSC · 43787-44241 · 455 bp

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Pi 0.0364

MarkerSeek score 75.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 47
pi: 0.0364
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	567-611 bp	1.0	75.7
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	568-612 bp	1.0	75.7
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	617-661 bp	1.0	75.4
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	618-662 bp	1.0	75.4
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	AGTTCCAATGCTACGCCTTC	45.0	50.0	56.4	58.3	569-613 bp	1.0	75.2