Hotspot

trnG-GCC-trnfM-CAU

LSC · 35742-35930 · 189 bp

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Pi 0.0743

MarkerSeek score 75.90

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 189
variable_sites: 38
pi: 0.0743
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	253-266 bp	1.0	88.5
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	254-267 bp	1.0	88.5
trnG-GCC-trnfM-CAU_p3	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	294-307 bp	1.0	88.0
trnG-GCC-trnfM-CAU_p4	AGAAGATGCGGGTTCGATTC	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.1	57.8	251-264 bp	1.0	87.3
trnG-GCC-trnfM-CAU_p5	GAGAAGATGCGGGTTCGATT	TGAGGTCACGGGTTCAAATC	50.0	50.0	58.1	57.8	252-265 bp	1.0	87.3