Hotspot

petN-psbM

LSC · 27577-28810 · 1234 bp

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Pi 0.0392

MarkerSeek score 76.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1234
variable_sites: 136
pi: 0.0392
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	CGGTTGGACAAAGAGGACAG	50.0	55.0	58.0	58.5	1694-1750 bp	1.0	87.2
petN-psbM_p2	AGTAAATCTCGCTTGGGCTG	CAGACGGTTGGACAAAGAGG	50.0	55.0	58.0	58.5	1698-1754 bp	1.0	87.1
petN-psbM_p3	AGTAAATCTCGCTTGGGCTG	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.0	58.5	1909-1974 bp	1.0	87.0
petN-psbM_p4	AGTAAATCTCGCTTGGGCTG	CTTCATCAGACGGTTGGACA	50.0	50.0	58.0	57.5	1704-1741 bp	0.583333	70.0
petN-psbM_p5	AGTAAATCTCGCTTGGGCTG	ACTTCATCAGACGGTTGGAC	50.0	50.0	58.0	57.5	1705-1742 bp	0.583333	70.0